For UV irradiation, the culture medium was removed from the Petri dish and the cells were irradiated with UVC nm at 0. Annu Rev Biochem. Demple B, Halbrook J. Hybridization signals were recorded by exposure on x-ray films or quantified by using imagequant Molecular Dynamics software. We also acknowledge the valuable technical help of Ms. After irradiation, the medium was added back to the cells for further incubation. San Francisco: Freeman; This observation supports our conclusion that increased resistance was due to activation of APE-1 that occurs transiently and is absent 24 to 36 h after HOCl treatment, when APE-1 went back down to the basal level. However, several unusual features of APE-1 activation are evident.
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As a DNA repair protein, the APE-1 polypeptide is expected to be localized in the cell nucleus, as was found to be the case in earlier studies 40 Specifically, ROS induce a variety of genes in E. More remarkably, the cell killing profile as a function of toxicant concentration was nearly identical for HeLa and V79 cells data not shown.
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Pricscilla K. APE-1 was visualized by indirect immunofluorescence.